Article

Accurate prediction in reconstructed spatial transcriptomes does not ensure valid biological discovery

2026-06-24

Abstract excerpt

Spatial transcriptomics is increasingly extended by computational reconstruction of unmeasured genes from matched single-cell RNA-sequencing references, enabling transcriptome-wide analyses from targeted or sparse assays. Yet downstream analyses typically treat reconstructed expression as experimentally observed, overlooking prediction error and latent spatial variation that can misattribute tissue architecture t...

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Literature Corpus work: 63437d15-e8b3-5f68-bd35-12ad1efbf968
DOI: 10.64898/2026.06.19.733432

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