Article

Denaturing and dNTPs reagents improve SARS-CoV-2 detection via single and multiplex RT-qPCR

2026-05-02

Abstract excerpt

Background The COVID-19 pandemic, caused by SARS-CoV-2, highlighted the need for accurate and scalable diagnostic tools such as RT-qPCR. However, false-negative results may occur due to viral mutations and RNA secondary structures within target regions. Methods High-performance computing (HPC) was used to compile SARS-CoV-2 genomic sequences from GenBank and GISAID and generate consensus sequences for primer and p...

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Literature Corpus work: 505ad208-e4f7-5b9f-8fda-7d2a792f005b
DOI: 10.12688/f1000research.109673.3

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