Article

A sensitive multiplex RT-qPCR assay to detect SARS-CoV-2 in respiratory samples

2025-04-01

Abstract excerpt

We developed an in-house RT-qPCR assay for the detection of SARS-CoV-2 from different sample types. Novel primer and probe sets were designed to amplify 2 regions of the nucleocapsid (N) gene. Two dual-target multiplex assays were then evaluated for performance in detection of SARS-CoV-2 in known samples. An assay combining one novel primer pair for N (N6), a published region for the envelope protein (E) and an ex...

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Literature Corpus work: 49d9d0a6-dbb3-54d8-a987-5d25a1d74560
DOI: 10.1101/2025.03.31.25323652

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