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Detection of SARS-CoV-2 RNA by multiplex RT-qPCR

7 Oct 2020

Abstract excerpt

The current quantitative reverse transcription PCR (RT-qPCR) assay recommended for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing in the United States requires analysis of 3 genomic targets per sample: 2 viral and 1 host. To simplify testing and reduce the volume of required reagents, we devised a multiplex RT-qPCR assay to detect SARS-CoV-2 in a single reaction. We used existing N1, N2, and...

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PMID: 33027248
DOI: 10.1371/journal.pbio.3000867

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