Article

Intracellular autofluorescence enables the isolation of viable, functional human muscle reserve cells with distinct Pax7 levels and stem cell states

2025-03-28

Abstract excerpt

<h4>Background</h4> Human muscle reserve cells (MuRC) represent a quiescent MuSC population generated in vitro that exhibit heterogeneous Pax7 expression, with a Pax7 High subset in a deeper quiescent state. However, conventional identification of Pax7 High cells requires intracellular staining, limiting their viability for functional studies. This study investigates autofluorescence (AF) as a potential biomar...

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Literature Corpus work: 4e195ddd-ac4b-5742-b797-de436b56932e
DOI: 10.1101/2025.03.28.645868

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