Article

Precise exome analysis of blastocyst biopsy scale samples using Primary Template directed Amplification

2025-06-09

Abstract excerpt

<title>Abstract</title> <p>This study evaluates primary template-directed amplification (PTA) for whole exome sequencing (WES) of small fibroblast cell groups, which mimics the limited cell quantities typical of trophectoderm embryo biopsies. PTA’s consistent amplification reduces allelic dropout (ADO) and improvesuniform coverage, overcoming challenges associated with conventional methods such as multiple displa...

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Literature Corpus work: 4c944418-2ef1-58ee-921a-81bb07bc8502
DOI: 10.21203/rs.3.rs-6745778/v1

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