Article

Pathotype identification of Leptosphaeria maculans with PCR and oligonucleotide primers from ribosomal internal transcribed spacer sequences

1992-09-01

Abstract excerpt

In order to develop a pathotype-specific assay, the internal transcribed spacer region 1 (ITS 1) of ribosomal RNA genes of highly and weakly virulent isolates of Leptosphaeria maculans were sequenced using primers from the flanking 17S and 5.8S ribosomal DNA (rDNA). The sequenced ITS 1 region had approximately 67% similarity among highly and weakly virulent isolates, and 37-46% similarity between L. maculans and o...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 48caafb0-5dd5-5e62-b657-7f82e265cf86
DOI: 10.1016/0885-5765(92)90009-k

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Pathotype identification of Leptosphaeria maculans with PCR and oligonucleotide primers from ribosomal internal transcribed spacer sequencesDOI 10.1016/0885-5765(92)90009-kRead

Comparison of the 5.8s rDNA and internal transcribed spacer sequences of isolates of Leptosphaeria maculans from different pathogenicity groups.Semantic proximity Read

Rapid identification of genetic variation and pathotype of Leptosphaeria maculans by random amplified polymorphic DNA assay.Semantic proximity Read

Estimating frequencies of virulent isolates in field populations of a plant pathogenic fungus, Leptosphaeria maculans, using high-throughput pyrosequencing.Semantic proximity Read

MPSeqM, a tool combining multiplex PCR and high-throughput sequencing to study the polymorphism of eight Leptosphaeria maculans avirulence genes and its application to field surveys in France.Semantic proximity Read

Rapid detection of Leptosphaeria maculans avirulence gene AvrLm4-7 conferring the avirulence/virulence specificity on Brassica napus using a tetra-primer ARMS-PCRSemantic proximity Read

Molecular characterisation and polymorphism of MinLm1, a minisatellite from the phytopathogenic ascomycete Leptosphaeria maculans.Semantic proximity Read

MPSeqM, a tool combining multiplex PCR and high-throughput sequencing to study the polymorphism of eight <i>Leptosphaeria maculans</i> avirulence genes and its application to field surveys in FranceSemantic proximity Read

Analysis of molecular markers genetically linked to the Leptosphaeria maculans avirulence gene AvrLm1 in field populations indicates a highly conserved event leading to virulence on Rlm1 genotypes.Semantic proximity Read

Genetic characterization of AvrLm1, the first avirulence gene of Leptosphaeria maculansSemantic proximity Read

Rep-PCR based genomic fingerprinting of isolates of Leptosphaeria maculans from PolandSemantic proximity Read

Detection and differentiation of plant-pathogenic mycoplasmalike organisms using polymerase chain reactionSemantic proximity Read

A 10-year survey of populations of Leptosphaeria maculans in France indicates a rapid adaptation towards the Rlm1 resistance gene of oilseed rapeSemantic proximity Read