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Rapid detection of Leptosphaeria maculans avirulence gene AvrLm4-7 conferring the avirulence/virulence specificity on Brassica napus using a tetra-primer ARMS-PCR

2018-10-01

Abstract excerpt

Leptosphaeria maculans is the causal agent of blackleg disease in canola (Brassica napus), resulting in significant yield loss in canola fields worldwide. AvrLm4-7 is an avirulence effector gene in L. maculans, and a single nucleotide mutation at codon 358 is responsible for the absence of the AvrLm4 allele. A tetra-primer amplification refractory mutation system-PCR assay (ARMS-PCR) was developed to rapidly diffe...

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Literature Corpus work
3aa53fe3-34df-5af3-a96c-51c191025b72
DOI
10.1007/s10658-018-1465-0
Open publication

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Rapid detection of Leptosphaeria maculans avirulence gene AvrLm4-7 conferring the avirulence/virulence specificity on Brassica napus using a tetra-primer ARMS-PCRDOI 10.1007/s10658-018-1465-0
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