Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Estimating frequencies of virulent isolates in field populations of a plant pathogenic fungus, Leptosphaeria maculans, using high-throughput pyrosequencing.

Journal of applied microbiology - 1 Nov 2012

Van de Wouw A P, Howlett B J

Abstract excerpt

AIM: To develop a pyrosequencing assay to monitor the frequency of alleles of an avirulence gene, AvrLm4, in populations of sexual spores of Leptosphaeria maculans, a fungal pathogen of canola (Brassica napus). METHODS AND RESULTS: The predominant mutation in AvrLm4 responsible for virulence to the corresponding resistance gene, Rlm4, is a single nucleotide polymorphism (SNP) at base 358. Pyrosequencing primers...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Ascomycota
Share to Brassica napus
Share to DNA, Fungal

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to High-Throughput Nucleotide Sequencing

Share to Plant Diseases

Share to Reproducibility of Results

Share to Spores, Fungal

Share to Virulence

Source

Identifiers

PMID: 22830361
DOI: 10.1111/j.1365-2672.2012.05413.x

Genes

IMPACT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Estimating frequencies of virulent isolates in field populations of a plant pathogenic fungus, Leptosphaeria maculans, using high-throughput pyrosequencing.PMID 22830361Read ↗

Determining frequencies of avirulent alleles in airborne Leptosphaeria maculans inoculum using quantitative PCRSemantic proximity Read ↗

MPSeqM, a tool combining multiplex PCR and high-throughput sequencing to study the polymorphism of eight Leptosphaeria maculans avirulence genes and its application to field surveys in France.Semantic proximity Read ↗

Identifying genetic diversity of avirulence genes in Leptosphaeria maculans using whole genome sequencing.Semantic proximity Read ↗

MPSeqM, a tool combining multiplex PCR and high-throughput sequencing to study the polymorphism of eight <i>Leptosphaeria maculans</i> avirulence genes and its application to field surveys in FranceSemantic proximity Read ↗

Rapid detection of Leptosphaeria maculans avirulence gene AvrLm4-7 conferring the avirulence/virulence specificity on Brassica napus using a tetra-primer ARMS-PCRSemantic proximity Read ↗

Molecular screening for avirulence alleles AvrLm1 and AvrLm6 in airborne inoculum of Leptosphaeria maculans and winter oilseed rape (Brassica napus) plants from Poland and the UK.Semantic proximity Read ↗

Evolution of linked avirulence effectors in Leptosphaeria maculans is affected by genomic environment and exposure to resistance genes in host plants.Semantic proximity Read ↗

Genetic diversity and population structure of Leptosphaeria maculans isolates in Western Canada.Semantic proximity Read ↗

Development of a specific marker for detection of a functional AvrLm9 allele and validating the interaction between AvrLm7 and AvrLm9 in Leptosphaeria maculans.Semantic proximity Read ↗

Genetic structure of a population of the fungus Leptosphaeria maculans in a disease nursery of Brassica napus in Australia.Semantic proximity Read ↗

Gene loss in the fungal canola pathogen Leptosphaeria maculans.Semantic proximity Read ↗

A game of hide and seek between avirulence genes AvrLm4-7 and AvrLm3 in Leptosphaeria maculans.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.