Article

Transcript-Capture sequencing enriches mRNA of <i>Mycobacterium tuberculosis</i> from host samples

2025-10-28

Abstract excerpt

<h4>ABSTRACT</h4> Bacterial gene expression from sites of infection are poorly studied due to low levels of bacterial mRNA present in clinical samples. Here, we develop Transcript-Capture Seq, which uses customizable biotinylated probes generated in-house to enrich bacteria-specific RNA from host samples before Next Generation Sequencing (NGS). This method results in a >200-fold increase in bacterial mRNA reads f...

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Literature Corpus work: 35c45e67-b283-5f1f-bc18-763759c3dc11
DOI: 10.1101/2025.10.28.685133

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