Article

40 minutes RT-qPCR Assay for Screening Spike N501Y and HV69-70del Mutations

2021-01-26

Abstract excerpt

<h4>ABSTRACT</h4> A one-step reverse transcription and real-time PCR (RT-qPCR) test was developed for rapid screening (40 minutes) of the Spike N501Y and HV69-70del mutations in SARS-CoV-2 positive samples. The test also targets a conserved region of SARS-CoV-2 Orf1ab as an internal control. The samples containing both the N501Y and HV69-70del mutations are concluded as VOC-202012/01 positive. Samples suspected t...

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Literature Corpus work: 34a86db8-9565-52e0-b624-e0cc07a3f465
DOI: 10.1101/2021.01.26.428302

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