Article

Higher efficiency of CRISPR/Cas9-mediated knock-in in HSV-2 by cleaving donor plasmid and viral target region simultaneously

2022-07-25

Abstract excerpt

The ability of engineering herpes simplex virus (HSV) underpins much in the reseach of its pathogeny biology and oncolytic virus therapy. Currently, the CRISPR/Cas9 system serves as the most powerful tool of HSV genome editing in human cells undoubtedly. Here, comparing the efficiency of gene insertion into HSV-2 based on CRISPR/Cas9-induced asymmetry and symmetry double-sided homology arms donor mediated knock-in...

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Literature Corpus work: 3285f9d9-9700-5442-9a0b-a844c93b337e
DOI: 10.21203/rs.3.rs-1888620/v1

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