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Article

One-step cross-genogroup multiplex RT-qPCR with an internal control system for the detection of infectious pancreatic necrosis virus (IPNV).

Journal of virological methods - 1 Sept 2017

Hoferer Marc, Braun Anne, Skrypski Julia, Bock Sabine, Thalheim Sabine, Sting Reinhard

Abstract excerpt

Infectious pancreatic necrosis virus (IPNV) causes great losses in fish hatcheries world-wide. The detection of IPNV can be challenging in certain circumstances, particularly due to low viral load and the genetic variability of this RNA virus. For the first time, this project created a quantitative triplex real-time reverse transcription PCR (RT-qPCR), including an endogenous control system, for specific,...

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PMID: 28559129
DOI: 10.1016/j.jviromet.2017.05.015

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