Article
Single-Tube Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Red-Spotted Grouper Nervous Necrosis Virus in Fish Species.
Viruses - 27 Jul 2026
Pradeep Mangottil Ayyappan, Subin Cherammpillil Sukumaran, Kumar Gokhlesh, Sharma Sulumane Ramachandra Krupesha, Sanil Nadiyath Karayi, Arun Kumar Thaliyil Veetil, Dhanutha Nikathil Raveendranathan, Shahansha Thevanattil Sairanksha Azhar, Vijayan Koyadan Kizhakkedath
Abstract excerpt
Betanodavirus is a causative agent of viral nervous necrosis (VNN) and a major threat to marine and brackish-water aquaculture globally. This virus causes epizootic outbreaks with particularly high morbidity and mortality in larval and juvenile stages and causes significant economic losses in aquaculture. Here, we developed a rapid and highly sensitive single-tube Reverse Transcription-Loop-Mediated Isothermal...
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