Article

RASP: Optimal single fluorescent puncta detection in complex cellular backgrounds

2023-12-18

Abstract excerpt

Super-resolution and single-molecule microscopy are increasingly applied to complex biological systems. A major challenge of this approach is that fluorescent puncta must be detected in the low signal, high noise, heterogeneous background environments of cells and tissue. We present RASP, Radiality Analysis of Single Puncta, a bioimaging-segmentation method that solves this problem. RASP removes false positive pun...

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Literature Corpus work: 241e0a4e-972d-56da-a150-1e1433906bde
DOI: 10.1101/2023.12.18.572148

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