Article

Co-expression of recombinant human collagen α1(III) chain with viral prolyl 4-Hydroxylase in <i>P. pastoris</i> GS115

2022-04-15

Abstract excerpt

Prolyl 4-hydroxylase (P4H) is essential to maintain the stable triple-helix structure and function of human collagen α1(Ш) chain (COL3A1). To obtain hydroxylated human COL3A1, the human COL3A1 and the viral P4H A085R were co-expressed in P. pastoris GS115. The sequence of human COL3A1 without N-terminal and C-terminal was selected for expression. Colony PCR analysis and sequencing after transfection showed tha...

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Literature Corpus work: 1ce37a60-9e23-52b3-882f-f759d059b7c2
DOI: 10.1101/2022.04.13.488258

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Co-expression of recombinant human collagen α1(III) chain with viral prolyl 4-Hydroxylase in <i>P. pastoris</i> GS115DOI 10.1101/2022.04.13.488258Read

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