Article

The challenge of distinguishing cell-cell complexes from singlet cells in non-imaging flow cytometry and single-cell sorting

2020-01-31

Abstract excerpt

Our recent work has highlighted that care needs to be taken when interpreting single cell data originating from flow cytometry acquisition or cell sorting: We found that doublets of T cells bound to other immune cells are often present in the live singlet gate of human peripheral blood samples acquired by flow cytometry. This hidden ‘contamination’ generates atypical gene signatures of mixed cell lineage in what i...

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Literature Corpus work: 1cc27d01-e949-5bcd-9ded-fbe00e93ac2f
DOI: 10.1101/2020.01.30.927137

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