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Tyramide Signal Amplification coupled with multiple immunolabeling and RNAscope in situ hybridization in formaldehyde-fixed paraffin-embedded human fetal brain.

2021-03-01

Abstract excerpt

<title>Abstract</title> <p>Several strategies have been recently introduced to improve the practicality of multiple immunolabelling and RNA <italic>in situ</italic> hybridization methods. We present a modified hybrid protocol of recently described complex detection strategies: (1) elution of antibodies prior to second round of staining (2) use of integrated polymers of HRP with secondary antibodies, and (3) tyram...

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Literature Corpus work
191aa65a-b37b-5757-b5a0-40f81e56a101
DOI
10.21203/rs.3.rs-183673/v1
Open publication

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Tyramide Signal Amplification coupled with multiple immunolabeling and RNAscope in situ hybridization in formaldehyde-fixed paraffin-embedded human fetal brain.DOI 10.21203/rs.3.rs-183673/v1
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