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Article

Characterization of hypothalamic neurons expressing a neuropeptide receptor, GALR2, using combined in situ hybridization-immunohistochemistry.

Methods (San Diego, Calif.) - 1 Aug 1999

Nichol K A, Depczynski B B, Cunningham A M

Abstract excerpt

Our laboratory is interested in characterizing the neurotransmitter and hormonal phenotype of neurons in the rat hypothalamus expressing novel neuropeptide receptors of the neuropeptide Y and galanin families. In this review, we describe a technique combining nonradioactive in situ hybridization to detect mRNA and fluorescence immunohistochemistry to detect protein antigens. We examined paraffin sections of rat...

Topics

Animals
Hypothalamus
Immunohistochemistry
In Situ Hybridization
Male
Microscopy, Confocal
Neurons
Phenotype
RNA, Messenger
Rats
Rats, Wistar

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Receptors, Galanin

Receptors, Neuropeptide

Sensitivity and Specificity

Somatostatin

Tyrosine 3-Monooxygenase

Source

Identifiers

PMID: 10491278
DOI: 10.1006/meth.1999.0816

Genes

GALR2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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