Article

A rapid and sensitive PCR-based assay for concurrent detection of bacteria causing common and halo blights in bean seed

1996-04-01

Abstract excerpt

A quick polymerase chain reaction (PCR)-based procedure was developed for concurrent detection of the causal agents of bean common blight, Xanthomonas campestris pv. phaseoli, and bean halo blight, Pseudomonas syringae pv. phaseolicola, in bean seed. A rapid DNA extraction procedure, consisting of briefly wetting intact or crushed seeds with a solution of sodium hydroxide, was used to extract bacterial DNA from ex...

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Literature Corpus work: 16ea2d85-1eee-5276-89c8-80b075818b51
DOI: 10.1094/phyto-86-361

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