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Detection of the bean common blight bacteria, Xanthomonas campestris pv. phaseoli and X. c. phaseoli var. fuscans, using the polymerase chain reaction

1994-10-01

Abstract excerpt

A 3.4-kb plasmid DNA fragment (p7) of Xanthomonas campestris pv. phaseoli that hybridizes specifically to X.c. phaseoli was subcloned and partially sequenced to design primers for a polymerase chain reaction (PCR) assay for the detection of bacteria causing common blight in bean. In Southern hybridization experiments, p7X3 and p7X4 subfragments showed high specificity for total genomic DNA of pathogenic X. c. phas...

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Literature Corpus work
c6e16d79-16a2-5239-b741-f125d92c4c1e
DOI
10.1094/phyto-84-1185
Open publication

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Detection of the bean common blight bacteria, Xanthomonas campestris pv. phaseoli and X. c. phaseoli var. fuscans, using the polymerase chain reactionDOI 10.1094/phyto-84-1185
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