Article

Real-time polymerase chain reaction: applications to studies on soilborne pathogens

2005-01-01

Abstract excerpt

Real-time, or quantitative, polymerase chain reaction (Q-PCR), offers a rapid, sensitive, and specific method for the diagnosis of plant pathogens in soil, water, air, and plant samples. Fluorescence is used to monitor the accumulation of the PCR product after each PCR cycle. The fluorescence data are used to extrapolate the amount of target DNA present in the sample before amplification so that detection and quan...

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Literature Corpus work: 0f5102ea-5de6-5165-abc3-ca48d9c3fafa
DOI: 10.1080/07060660509507229

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