Article

Hollow-fibre biomanufacturing and cell-free engineering of HEK293 extracellular vesicles

2025-09-21

Abstract excerpt

Extracellular vesicles (EVs) are lipid-delineated nanoparticles that are produced by most cell types. EVs contain complex molecular cargoes that can have useful therapeutic or vaccine immunological effects. Cell-free gene expression systems can be used to produce membrane proteins in vitro , that can co-localise and integrate with exogenously added EVs. To advance this type of cell-free EV engineering we manufact...

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Literature Corpus work: 1150dce2-451b-5a2b-af34-d177e326c997
DOI: 10.1101/2025.09.20.677537

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