Article

CellSpecks: A Software for Automated Detection and Analysis of Calcium Channels in Live Cells

2018-06-25

Abstract excerpt

<h4>ABSTRACT</h4> To couple the fidelity of patch-clamp recording with a more high-throughput screening capability, we pioneered a novel approach to single channel recording that we named “optical patch clamp”. By using highly-sensitive fluorescent Ca 2+ indicator dyes in conjunction with total internal fluorescence microscopy techniques, we monitor Ca 2+ flux through individual Ca 2+ -permeable channels. This...

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Literature Corpus work: 0d1c9f0b-ce7c-5eaa-88d1-be9e85a54a05
DOI: 10.1101/355693

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