Back to search

Article

Establishment of a well-characterized SARS-CoV-2 lentiviral pseudovirus neutralization assay using 293T cells with stable expression of ACE2 and TMPRSS2

2020-12-26

Abstract excerpt

Pseudoviruses are useful surrogates for highly pathogenic viruses because of their safety, genetic stability, and scalability for screening assays. Many different pseudovirus platforms exist, each with different advantages and limitations. Here we report our efforts to optimize and characterize an HIV-based lentiviral pseudovirus assay for screening neutralizing antibodies for SARS-CoV-2 using a stable 293T cell l...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
0a5aa082-24b4-5cd6-80fb-21d978d4d9be
DOI
10.1101/2020.12.26.424442
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Establishment of a well-characterized SARS-CoV-2 lentiviral pseudovirus neutralization assay using 293T cells with stable expression of ACE2 and TMPRSS2DOI 10.1101/2020.12.26.424442
Establishment of a well-characterized SARS-CoV-2 lentiviral pseudovirus neutralization assay using 293T cells with stable expression of ACE2 and TMPRSS2Semantic proximity
SARS-CoV-2 Spike Pseudoviruses: A Useful tool to study virus entry and address emerging neutralization escape phenotypesSemantic proximity
A Pseudovirus-Based Neutralization Assay for SARS-CoV-2 Variants: A Rapid, Cost-Effective, BSL-2–Based High-Throughput Assay Useful for Vaccine Immunogenicity EvaluationSemantic proximity
Quantifying absolute neutralization titers against SARS-CoV-2 by a standardized virus neutralization assay allows for cross-cohort comparisons of COVID-19 seraSemantic proximity
Neutralizing antibody against SARS-CoV-2 spike in COVID-19 patients, health care workers and convalescent plasma donors: a cohort study using a rapid and sensitive high-throughput neutralization assaySemantic proximity
A nanoluciferase SARS-CoV-2 for rapid neutralization testing and screening of anti-infective drugs for COVID-19Semantic proximity
Human Immunodeficiency Viruses Pseudotyped with SARS-CoV-2 Spike Proteins Infect a Broad Spectrum of Human Cell Lines through Multiple Entry MechanismsSemantic proximity
ACE2-Coated Virus-Like Particles Effectively Block SARS-CoV-2 InfectionSemantic proximity
SARS CoV-2 Nucleoprotein Enhances the Infectivity of Lentiviral Spike ParticlesSemantic proximity
A multiplexed high-throughput neutralization assay reveals a lack of activity against multiple variants after SARS-CoV-2 infectionSemantic proximity
SARS CoV-2 nucleoprotein enhances the infectivity of lentiviral spike particlesSemantic proximity
Developing Pseudovirus-Based Neutralization Assay against Omicron-Included SARS-CoV-2 VariantsSemantic proximity
Select a neighboring publication to make it the new centre.