Article

Nanopore quality score resolution can be reduced with little effect on downstream analysis

2022-03-05

Abstract excerpt

We investigate the effect of quality score information loss on downstream analysis from nanopore sequencing FASTQ files. We polished denovo assemblies for a mock microbial community and a human genome, and we called variants on a human genome. We repeated these experiments using various pipelines, under various coverage level scenarios, and various quality score quantizers. In all cases we found that the quantizat...

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Literature Corpus work: 04cf3d51-be6e-5430-989d-2944f5eda3a0
DOI: 10.1101/2022.03.03.482048

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