Article
Dual-color flow cytometric analysis of phenotype, activation marker expression, and proliferation of mitogen-stimulated bovine lymphocyte subsets.
Veterinary immunology and immunopathology - 4 Jan 1999
Quade M J, Roth J A
Abstract excerpt
Bovine peripheral blood mononuclear cells were cultured in vitro for 3 days with the mitogens concanavalin A (Con A), pokeweed mitogen (PWM), phytohemagglutinin (PHA), and anti-CD3 monoclonal antibody. Activation of T-lymphocyte subsets (CD4+, CD8+, and gammadelta T-cell receptor+) and of B-cells...
Topics
- Animals
- CD3 Complex
- Cattle
- Cell Division
- Cell Separation
- Concanavalin A
- Flow Cytometry
- Histocompatibility Antigens Class II
- Lymphocyte Activation
- Lymphocyte Subsets
- Mitogens
- Phenotype
- Phytohemagglutinins
- Pokeweed Mitogens
- Receptors, Interleukin-2
- Surface Properties