Article
Characterization of the bleomycin resistance determinant encoded on the transposon Tn5.
FEBS letters - 8 Jan 1999
Kumagai T, Nakano T, Maruyama M, Mochizuki H, Sugiyama M
Abstract excerpt
The transposon Tn5 carries a gene, ble, which confers resistance to bleomycin (Bm) and gives a survival advantage to its host cell. We found that the ble gene product, designated BLMT, is a binding protein with a strong affinity for Bm. BLMT quenched both the antibacterial and DNA-cleaving activi...
Topics
- Acetyltransferases
- Actinomycetales
- Amino Acid Sequence
- Anti-Bacterial Agents
- Bacterial Proteins
- Base Sequence
- Bleomycin
- Carrier Proteins
- Chemical Phenomena
- Chemistry, Physical
- DNA Primers
- DNA Transposable Elements
- Drug Resistance, Microbial
- Electron Spin Resonance Spectroscopy
- Escherichia coli
- Immunochemistry
- Molecular Sequence Data
- Mutagenesis, Site-Directed