Article
Bleomycin-resistance gene derived from the transposon Tn5 confers selective advantage to Escherichia coli K-12.
Proceedings of the National Academy of Sciences of the United States of America - 15 Oct 1991
Blot M, Meyer J, Arber W
Abstract excerpt
The plasmid pRAB2 contains a silent operon derived from the transposon Tn5 and carrying the gene neo for neomycin-kanamycin resistance and a truncated ble gene (ble333) for bleomycin resistance. Spontaneous mutants that express the two resistances provide Escherichia coli cells an improved fitnes...
Topics
- Bleomycin
- Cell Death
- Chromosome Deletion
- Cloning, Molecular
- DNA Transposable Elements
- Drug Resistance, Microbial
- Escherichia coli
- Genes, Bacterial
- Mutation
- Operon
- Phenotype
- R Factors
- Restriction Mapping