Article
Instability of pUC19 in Escherichia coli transcription termination factor mutant, rho026.
Plasmid - 1 Jan 1999
Sozhamannan S, Morris J G, Stitt B L
Abstract excerpt
The higher copy number of pUC19, compared to its parent plasmid pBR322, is known to be due to deletion of rop, also known as rom, and to an ori mutation that impedes RNAI:RNAII interaction. pUC19, unlike pBR322, fails to transform E. coli rho mutant rho026 cells. Here we identify two features of...
Topics
- Bacterial Proteins
- DNA Replication
- Endoribonucleases
- Escherichia coli
- Gene Dosage
- Mutation
- Plasmids
- RNA Polymerase I
- RNA Polymerase II
- Rho Factor
- Transformation, Bacterial