Article
Activity and cellular location in Saccharomyces cerevisiae of chimeric mouse/yeast and Bacillus subtilis/yeast ferrochelatases.
Archives of biochemistry and biophysics - 15 Jan 1999
Góra M, Rytka J, Labbe-Bois R
Abstract excerpt
We have constructed a series of chimeric yeast/mouse and yeast/Bacillus subtilis ferrochelatase genes in order to investigate domains of the ferrochelatase that are important for activity and/or association with the membrane. These genes were expressed in a Saccharomyces cerevisiae mutant in whic...
Topics
- Amino Acid Sequence
- Animals
- Bacillus subtilis
- Cell Membrane
- Enzyme Activation
- Ferrochelatase
- Mice
- Molecular Sequence Data
- Peptide Fragments
- Phenotype
- Protein Sorting Signals
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Sequence Deletion
- Sequence Homology, Amino Acid