Article
Mutations in a peptidylprolyl-cis/trans-isomerase gene lead to a defect in 3'-end formation of a pre-mRNA in Saccharomyces cerevisiae.
The Journal of biological chemistry - 1 Jan 1999
Hani J, Schelbert B, Bernhardt A, Domdey H, Fischer G, Wiebauer K, Rahfeld J U
Abstract excerpt
In a genetic screen aimed at the identification of trans-acting factors involved in mRNA 3'-end processing of budding yeast, we have previously isolated two temperature-sensitive mutants with an apparent defect in the 3'-end formation of a plasmid-derived pre-mRNA. Surprisingly, both mutants were rescued by the essential gene ESS1/PTF1 that encoded a putative peptidylprolyl-cis/trans-isomerase (PPIase) (Hani, J.,...
Topics
- Amino Acid Isomerases
- Amino Acid Sequence
- Base Sequence
- Blotting, Western
- DNA Primers
- Molecular Sequence Data
- Mutation
- NIMA-Interacting Peptidylprolyl Isomerase
- Peptidylprolyl Isomerase
- Phenotype
- RNA Precursors
- RNA Processing, Post-Transcriptional