Article
Protein component of the ribozyme ribonuclease P alters substrate recognition by directly contacting precursor tRNA.
Proceedings of the National Academy of Sciences of the United States of America - 22 Dec 1998
Niranjanakumari S, Stams T, Crary S M, Christianson D W, Fierke C A
Abstract excerpt
The protein component of ribonuclease P (RNase P) binds to the RNA subunit, forming a functional ribonucleoprotein complex in vivo and enhancing the affinity of the precursor tRNA (pre-tRNA) substrate. Photocrosslinking experiments with pre-tRNA bound to RNase P reconstituted with the protein com...
Topics
- Amino Acid Substitution
- Base Sequence
- Catalytic Domain
- Computer Simulation
- Cysteine
- DNA Primers
- Endoribonucleases
- Genetic Variation
- Models, Molecular
- Mutagenesis, Site-Directed
- Polymerase Chain Reaction
- Protein Structure, Secondary
- RNA Precursors
- RNA, Catalytic