Article
Splice donor site mutations in the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene cause a deficiency of the endoplasmic reticulum 3-hydroxy-3-methylglutaryl coenzyme A reductase protein in UT2 cells.
Journal of lipid research - 1 Nov 1998
Engfelt W H, Masuda K R, Paton V G, Krisans S K
Abstract excerpt
UT2 cells are a mutant clone of Chinese hamster ovary (CHO) cells that are deficient in the 97 kDa endoplasmic reticulum (ER) 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase protein. The analysis of UT2 cell cDNA and genomic DNA has led to the identification of two novel point mutations...
Topics
- Alleles
- Animals
- Blotting, Northern
- Blotting, Southern
- CHO Cells
- Cricetinae
- DNA, Complementary
- Endoplasmic Reticulum
- Exons
- Hydroxymethylglutaryl CoA Reductases
- Molecular Weight
- Mutation
- Polymorphism, Restriction Fragment Length
- RNA Splicing
- Sequence Analysis, DNA