Article
Application of RSCA for the typing of HLA-DPB1.
Human immunology - 1 Nov 1998
Ramon D S, Argüello J R, Cox S T, McWhinnie A, Little A M, Marsh S G, Madrigal J A
Abstract excerpt
We describe the application of RSCA, for the high resolution typing of alleles encoded at the HLA-DPB1 locus. RSCA differs from other sequence based typing methodologies in that the HLA type is assigned on the basis of differences in DNA conformation between different alleles. A total of 251 samp...
Topics
- Alleles
- Cell Line
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Genes, MHC Class II
- Genetic Techniques
- HLA-DP Antigens
- HLA-DP beta-Chains
- Heterozygote
- Histocompatibility Testing
- Humans
- Molecular Weight
- Nucleic Acid Conformation
- Nucleic Acid Hybridization
- Point Mutation
- Polymerase Chain Reaction
- Reproducibility of Results
- Sensitivity and Specificity