Article
Activation of cAMP-dependent C1- currents in guinea-pig paneth cells without relevant evidence for CFTR expression.
The Journal of physiology - 1 Nov 1998
Tsumura T, Hazama A, Miyoshi T, Ueda S, Okada Y
Abstract excerpt
1. To determine whether Paneth cells exhibit functional expression of cAMP-activated Cl- currents and molecular expression of the cystic fibrosis transmembrane conductance regulator (CFTR), we applied whole-cell patch clamp and single-cell mRNA analysis by reverse transcription (RT) followed by polymerase chain reaction (PCR) amplification to single Paneth cells in crypts isolated from the guinea-pig small...
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