Article
Identification of mouse crystallins in 2D protein patterns by sequencing and mass spectrometry. Application to cataract mutants.
FEBS letters - 18 Sept 1998
Jungblut P R, Otto A, Favor J, Löwe M, Müller E C, Kastner M, Sperling K, Klose J
Abstract excerpt
The eye lens proteins of the mouse were separated into 1940 polypeptide spots by two-dimensional electrophoresis in large gels. All 16 crystallins ubiquitous in mammals were identified by protein sequencing and mass spectrometry except for (gamma)-F, which shows an almost identical sequence with (gamma)-E. Two crystallins, (beta)-A2 and (gamma)-S, were shown for the first time to occur in the mouse lens. An...
Topics
- Amino Acid Sequence
- Animals
- Cataract
- Crystallins
- Electrophoresis, Gel, Two-Dimensional
- Mass Spectrometry
- Mice
- Molecular Sequence Data
- Mutation
- Peptides
- Sequence Analysis