Article
An inactive pancreatic lipase-related protein is activated into a triglyceride-lipase by mutagenesis based on the 3-D structure.
Chemistry and physics of lipids - 1 Jun 1998
Bezzine S, Roussel A, de Caro J, Gastinel L, de Caro A, Carrière F, Leydier S, Verger R, Cambillau C
Abstract excerpt
Both classical dog pancreatic lipase (DPL) and dog pancreatic lipase-related protein 1 (DPLRP1) have been found to be secreted by the exocrine pancreas. These two proteins were purified to homogeneity from canine pancreatic juice and no significant catalytic activity was observed with DPLRP1 on a...
Topics
- Amino Acid Sequence
- Animals
- Crystallography, X-Ray
- Dogs
- Enzyme Activation
- Kinetics
- Lipase
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Pancreatic Juice
- Protein Conformation
- Structure-Activity Relationship
- Triglycerides