Article
The upstream sequence element of the C2 complement poly(A) signal activates mRNA 3' end formation by two distinct mechanisms.
Genes & development - 15 Aug 1998
Moreira A, Takagaki Y, Brackenridge S, Wollerton M, Manley J L, Proudfoot N J
Abstract excerpt
The poly(A) signal of the C2 complement gene is unusual in that it possesses an upstream sequence element (USE) required for full activity in vivo. We describe here in vitro experiments demonstrating that this USE enhances both the cleavage and poly(A) addition reactions. We also show that the C2...
Topics
- Binding Sites
- Complement C2
- Humans
- Mutation
- Poly A
- Polypyrimidine Tract-Binding Protein
- Promoter Regions, Genetic
- RNA Splicing
- RNA, Messenger
- RNA-Binding Proteins
- Recombinant Proteins
- Ribonucleoproteins
- Transcriptional Activation
- mRNA Cleavage and Polyadenylation Factors