Article
Detection of known and new mutations in the thiopurine S-methyltransferase gene by single-strand conformation polymorphism analysis.
Human mutation - 1 Jan 1998
Spire-Vayron de la Moureyre C, Debuysère H, Sabbagh N, Marez D, Vinner E, Chevalier E D, Lo Guidice J M, Broly F
Abstract excerpt
To detect mutations in the thiopurine S-methyltransferase gene (TPMT), we have developed a strategy based on single-strand conformation polymorphism (SSCP) analysis of the gene amplified by polymerase chain reaction (PCR). The sensitivity of the method was first evaluated by analyzing DNA samples...
Topics
- Alleles
- Genotype
- Humans
- Methyltransferases
- Mutation
- Phenotype
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational