Article
Detection of known and new mutations in the thiopurine S-methyltransferase gene by single-strand conformation polymorphism analysis.
Human mutation - 1 Jan 1998
Spire-Vayron de la Moureyre C, Debuysère H, Sabbagh N, Marez D, Vinner E, Chevalier E D, Lo Guidice J M, Broly F
Abstract excerpt
To detect mutations in the thiopurine S-methyltransferase gene (TPMT), we have developed a strategy based on single-strand conformation polymorphism (SSCP) analysis of the gene amplified by polymerase chain reaction (PCR). The sensitivity of the method was first evaluated by analyzing DNA samples from five individuals, including two high methylators (HMs), two intermediate methylators (IMs), and one deficient...
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