Capillary electrophoretic analysis of DNA restriction fragments and PCR products for polymorphism and mutation studies in cystic fibrosis and Gaucher's disease.

Abstract excerpt

Two methods are described for the analysis of DNA restriction fragments and PCR products in studies on polymorphism and mutation in cystic fibrosis and Guacher's disease, based on capillary electrophoresis. In one CE system, a Beckman kit for producing a chemical gel (polymerized within the capil...

Topics

• Cystic Fibrosis
• DNA
• Electrophoresis, Polyacrylamide Gel
• Exons
• Gaucher Disease
• Humans
• Indicators and Reagents
• Leukocytes
• Mutation
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Restriction Mapping