Article
Detection of the R553X DNA single point mutation related to cystic fibrosis by a "chiral box" D-lysine-peptide nucleic acid probe by capillary electrophoresis.
Electrophoresis - 1 Nov 2005
Tedeschi Tullia, Chiari Marcella, Galaverna Gianni, Sforza Stefano, Cretich Marina, Corradini Roberto, Marchelli Rosangela
Abstract excerpt
In order to recognize the presence of the R553X point mutation of the cystic fibrosis (CF) gene in the human genome, a peptide nucleic acid (PNA) complementary to the mutated gene tract and bearing three adjacent chiral monomers based on D-lysine (chiral box) was synthesized and used as a probe in CE. Binding specificity was preliminarily studied with complementary and mismatched oligonucleotides by UV...
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