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Article

Analysis of polymerase chain reaction product by capillary electrophoresis and its application to the detection of single base substitution in genes.

Journal of chromatography. A - 25 Mar 1994

Arakawa H, Uetanaka K, Maeda M, Tsuji A

Abstract excerpt

Capillary gel electrophoresis (CGE) was studied for the direct analysis of polymerase chain reaction (PCR) amplified samples. A low cross-linked polyacrylamide gel (3%T, 0.5%C) was used for CGE with treated and untreated silica capillaries. CGE showed high reproducibility and resolution in the separation of DNA fragments (ca. 100-1000 base pairs) produced by PCR. The CGE system was applied to the detection of an...

Topics

Bacteriophage phi X 174
DNA Mutational Analysis
DNA, Viral
Deoxyribonucleases, Type II Site-Specific
Electrophoresis, Polyacrylamide Gel
Humans
Mutation
Phenylalanine Hydroxylase
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 7912142
DOI: 10.1016/0021-9673(94)80632-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7912142-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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