Article
Analysis of polymerase chain reaction product by capillary electrophoresis and its application to the detection of single base substitution in genes.
Journal of chromatography. A - 25 Mar 1994
Arakawa H, Uetanaka K, Maeda M, Tsuji A
Abstract excerpt
Capillary gel electrophoresis (CGE) was studied for the direct analysis of polymerase chain reaction (PCR) amplified samples. A low cross-linked polyacrylamide gel (3%T, 0.5%C) was used for CGE with treated and untreated silica capillaries. CGE showed high reproducibility and resolution in the separation of DNA fragments (ca. 100-1000 base pairs) produced by PCR. The CGE system was applied to the detection of an...
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