Article
Rearrangement reactions in the biosynthesis of molybdopterin--an NMR study with multiply 13C/15N labelled precursors.
European journal of biochemistry - 1 Jul 1998
Rieder C, Eisenreich W, O'Brien J, Richter G, Götze E, Boyle P, Blanchard S, Bacher A, Simon H
Abstract excerpt
The genes moaABC of Escherichia coli were ligated into the expression vector pNCO113. The resulting plasmid was transformed into a moeA mutant of E. coli. From cultures of the recombinant strain, a pteridine designated compound Z could be isolated at 5 mg/liter. Compound Z is a product of precurs...
Topics
- Carbon Isotopes
- Coenzymes
- Escherichia coli
- Escherichia coli Proteins
- Glucose
- Guanine
- Isotope Labeling
- Metalloproteins
- Models, Chemical
- Molybdenum
- Molybdenum Cofactors
- Mutation
- Nitrogen Isotopes
- Nuclear Magnetic Resonance, Biomolecular
- Pteridines