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Article

Production of soluble αβ T‐cell receptor heterodimers suitable for biophysical analysis of ligand binding

1 Jan 1999

Abstract excerpt

A method to produce alphabeta T-cell receptors (TCRs) in a soluble form suitable for biophysical analysis was devised involving in vitro refolding of a TCR fusion protein. Polypeptides corresponding to the variable and constant domains of each chain of a human and a murine receptor, fused to a coiled coil heterodimerization motif from either c-Jun (alpha) or v-Fos (beta), were overexpressed separately in...

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Identifiers

PMID: 10595544
DOI: 10.1110/ps.8.11.2418

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Source receipt: pubmed, retrieved 7 Sept 2026.

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