Facile construction of mutations in Haemophilus ducreyi using lacZ as a counter-selectable marker.

doi:10.1111/j.1574-6968.1998.tb13097.x

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Article

Facile construction of mutations in Haemophilus ducreyi using lacZ as a counter-selectable marker.

FEMS microbiology letters - 15 Jul 1998

Bozue J A, Tarantino L, Munson R S

Abstract excerpt

Haemophilus ducreyi is a Gram-negative bacterium which is the causative agent of chancroid, an ulcerative sexually transmitted disease. In order to understand the pathogenesis of H. ducreyi disease, studies designed to identify potential virulence determinants and construct mutants deficient in t...

Topics

Alleles
Electroporation
Genes, Reporter
Genetic Vectors
Haemophilus ducreyi
Hemolysin Proteins
Humans
Lac Operon
Mutation
Plasmids
beta-Galactosidase

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