Article
Gene inactivation in Coxiella burnetii.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Beare Paul A, Heinzen Robert A
Abstract excerpt
Coxiella burnetii, the agent of human Q fever, is a zoonotic bacterial pathogen with a worldwide distribution. Owing to an historic lack of methods for genetic manipulation, virulence factors deployed by this bacterium for disease pathogenesis are poorly understood. However, the recent advance of host cell-free (axenic) growth of C. burnetii has coincided with development of several new genetic technologies...
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