Article
The broad specificity of dominant inhibitory protein kinase C mutants infers a common step in phosphorylation.
The Biochemical journal - 1 Aug 1998
Garcia-Paramio P, Cabrerizo Y, Bornancin F, Parker P J
Abstract excerpt
Dominant negative properties are conferred on protein kinase (PK) Calpha by mutation of the phosphorylation site in the activation loop of the kinase domain. To address the universality and/or specificity of such mutations, analogous alterations were introduced in other members of the PKC family...
Topics
- Animals
- Binding Sites
- Blotting, Western
- COS Cells
- Cattle
- Isoenzymes
- Mice
- Mutation
- Phosphorylation
- Protein Kinase C
- Rats
- Substrate Specificity
- Transfection