Article
Interaction of oxyimino beta-lactams with a class C beta-lactamase and a mutant with a spectrum extended to beta-lactams.
Antimicrobial agents and chemotherapy - 1 Jun 1994
Nukaga M, Tsukamoto K, Yamaguchi H, Sawai T
Abstract excerpt
The class C beta-lactamase of Citrobacter freundii GN346 is a typical cephalosporinase comprising 361 amino acids, and substitution of the glutamic acid at position 219 in the enzyme by lysine was previously shown to broaden its substrate spectrum to oxyimino beta-lactams (K. Tsukamoto, R. Ohno, and T. Sawai, J. Bacteriol. 172:4348-4351, 1990). To clarify this spectrum extension from the kinetic point of view,...
Topics
- Anti-Bacterial Agents
- Aztreonam
- Kinetics
- Mutation
- beta-Lactamase Inhibitors