Article
Rapid screening for penicillin susceptibility of systemic pneumococcal isolates by restriction enzyme profiling of the pbp2B gene.
Journal of clinical microbiology - 1 Aug 1998
Beall B, Facklam R R, Jackson D M, Starling H H
Abstract excerpt
Restriction digest profiling of pneumococcal pbp2b-specific amplicons was effective for screening penicillin resistance. The pbp2b amplicon of all pneumococcal isolates for which the MICs of penicillin were < or = 0.03 microgram/ml had one of two different susceptible restriction profiles, and al...
Topics
- Alleles
- Aminoacyltransferases
- Bacterial Proteins
- Base Sequence
- Carrier Proteins
- DNA Fingerprinting
- Deoxyribonucleases, Type II Site-Specific
- Female
- Hexosyltransferases
- Humans
- Male
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Muramoylpentapeptide Carboxypeptidase
- Penicillin Resistance
- Penicillin-Binding Proteins
- Penicillins
- Peptidyl Transferases